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vitro cell viability human aortic endothelial cells haec  (PromoCell)


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    PromoCell vitro cell viability human aortic endothelial cells haec
    Vitro Cell Viability Human Aortic Endothelial Cells Haec, supplied by PromoCell, used in various techniques. Bioz Stars score: 96/100, based on 233 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/vitro cell viability human aortic endothelial cells haec/product/PromoCell
    Average 96 stars, based on 233 article reviews
    vitro cell viability human aortic endothelial cells haec - by Bioz Stars, 2026-02
    96/100 stars

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    (A) Differential transcript expression following in <t>vitro</t> <t>human</t> <t>aortic</t> <t>endothelial</t> cells stimulation with IL-17 versus PBS, IL-17+TNF-α versus PBS, IL-17+IFN-γ versus PBS (p<0.05 for all changes). (B) Direct analysis of venous endothelial cells harvested from psoriasis patients compared to controls show transcript upregulation in intracellular adhesion molecules (VCAM-1, ICAM-1), inflammation (COX-2) as well as chemokines (CXCL10, CXCL1, CX3CL1, CCL3, MCP-1) interleukins and tumor necrosis factors (IL-1β, IL-8, Lymphotoxin beta). (C) Differential endothelial transcript expression in psoriasis over controls stratified by psoriasis severity (control, PASI: ≤ 10, > 10). (D) Endothelial inflammatory activation in psoriatic patients, as assessed by nuclear p65 NFκB localization in the vascular endothelium of brachial vein endothelial cells. Direct immunofluorescence staining of harvested venous endothelial cells (VE-cadherin, green) of psoriasis patients compared to healthy controls show increased p65 NFκB (red) nuclear (DAPI - blue) co-localization (n=6). p<0.05*, p<0.01**. DAPI, 4’,6-diamidino-2-phenylinodle; IL, interleukin; NFκB, Nuclear factor kappa-light-chain-enhancer of activated B cells; PASI, psoriasis area and severity index; PSB, phosphate buffered-saline; TNF, tumor necrosis factor; VEGFA, vascular endothelial growth factor A; vWF, von willebrand factor.
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    (A) Differential transcript expression following in vitro human aortic endothelial cells stimulation with IL-17 versus PBS, IL-17+TNF-α versus PBS, IL-17+IFN-γ versus PBS (p<0.05 for all changes). (B) Direct analysis of venous endothelial cells harvested from psoriasis patients compared to controls show transcript upregulation in intracellular adhesion molecules (VCAM-1, ICAM-1), inflammation (COX-2) as well as chemokines (CXCL10, CXCL1, CX3CL1, CCL3, MCP-1) interleukins and tumor necrosis factors (IL-1β, IL-8, Lymphotoxin beta). (C) Differential endothelial transcript expression in psoriasis over controls stratified by psoriasis severity (control, PASI: ≤ 10, > 10). (D) Endothelial inflammatory activation in psoriatic patients, as assessed by nuclear p65 NFκB localization in the vascular endothelium of brachial vein endothelial cells. Direct immunofluorescence staining of harvested venous endothelial cells (VE-cadherin, green) of psoriasis patients compared to healthy controls show increased p65 NFκB (red) nuclear (DAPI - blue) co-localization (n=6). p<0.05*, p<0.01**. DAPI, 4’,6-diamidino-2-phenylinodle; IL, interleukin; NFκB, Nuclear factor kappa-light-chain-enhancer of activated B cells; PASI, psoriasis area and severity index; PSB, phosphate buffered-saline; TNF, tumor necrosis factor; VEGFA, vascular endothelial growth factor A; vWF, von willebrand factor.

    Journal: Arteriosclerosis, thrombosis, and vascular biology

    Article Title: Inflammasome Signaling and Impaired Vascular Health in Psoriasis

    doi: 10.1161/ATVBAHA.118.312246

    Figure Lengend Snippet: (A) Differential transcript expression following in vitro human aortic endothelial cells stimulation with IL-17 versus PBS, IL-17+TNF-α versus PBS, IL-17+IFN-γ versus PBS (p<0.05 for all changes). (B) Direct analysis of venous endothelial cells harvested from psoriasis patients compared to controls show transcript upregulation in intracellular adhesion molecules (VCAM-1, ICAM-1), inflammation (COX-2) as well as chemokines (CXCL10, CXCL1, CX3CL1, CCL3, MCP-1) interleukins and tumor necrosis factors (IL-1β, IL-8, Lymphotoxin beta). (C) Differential endothelial transcript expression in psoriasis over controls stratified by psoriasis severity (control, PASI: ≤ 10, > 10). (D) Endothelial inflammatory activation in psoriatic patients, as assessed by nuclear p65 NFκB localization in the vascular endothelium of brachial vein endothelial cells. Direct immunofluorescence staining of harvested venous endothelial cells (VE-cadherin, green) of psoriasis patients compared to healthy controls show increased p65 NFκB (red) nuclear (DAPI - blue) co-localization (n=6). p<0.05*, p<0.01**. DAPI, 4’,6-diamidino-2-phenylinodle; IL, interleukin; NFκB, Nuclear factor kappa-light-chain-enhancer of activated B cells; PASI, psoriasis area and severity index; PSB, phosphate buffered-saline; TNF, tumor necrosis factor; VEGFA, vascular endothelial growth factor A; vWF, von willebrand factor.

    Article Snippet: In vitro human aortic endothelial methods and analysis: Human aortic endothelial cells (HAECs) were cultured in Endothelial Cell Growth Medium MV 2 (Promocell® GmbH) at passage three.

    Techniques: Expressing, In Vitro, Activation Assay, Immunofluorescence, Staining